Fa'apolofesa Design High Quality Proteinase K CAS Numera 39450-01-6 fa'atasi ai ma le tau sili
E masani ona matou faʻatinoina le avea o se tagata faigaluega faʻapitoa ma mautinoa o le a matou tuʻuina atu ia te oe le sili ona aoga sili faʻatasi ai ma le tau faʻatau sili ona lelei mo Professional Design High QualityProteinase K CAS Numera 39450-01-6ma le Tau Sili, Mo faʻamatalaga faaopoopo ma mea moni, faʻamolemole talanoa mai ia i matou i se taimi vave e mafai ai!
E masani ona matou faʻatinoina le avea o se tagata faigaluega faʻapitoa ma mautinoa o le a matou tuʻuina atu ia te oe le sili sili ona aoga faʻatasi ai ma le tau faʻatau sili atu moSaina Tritirachium Album Limber ma Molecular Biology, Pulea lelei maoti e faʻatinoina i soʻotaga taʻitasi o le gaosiga atoa o le gaosiga. Matou te faʻamoemoe faamaoni e faʻavae le felagolagomai faauo ma fefaʻasoaaʻi faʻatasi ma oe. Faʻavae i luga o fofo maualuga ma atoatoa lelei muamua-faʻatau / ina ua uma-faʻatau auaunaga o lo matou manatu, o nisi tagata faʻatau na galulue faʻatasi ma i matou mo le silia ma le 5 tausaga.
| Nu.1 vai | |
| Gaoioiga faʻapitoa Polotini> 800U / mL, o le faʻatonuga o le 20mg / mL, leai ni Nickase toega | |
| Nu.2 pauta | |
| Fa'aaliga | Pa'epa'e lyophilized pauta |
| Electrophoretic mama | ≥95% |
| Gaioiga ole enzyme | ≥30U/mg |
| Nucleic acid toega | Le vaaia |
| Deoxyribonuclease toega | Le vaaia |
| Ribonuclease toega | Le vaaia |
| Fa'ato'aga Nu. | G1205-10ML |
| Teuina | Pe a teuina i le -20 °C, o le oloa e taofia le gaioiga mo le itiiti ifo i le 1 tausaga, felauaiga taga aisa. |
Proteinase Ko se serine protease o loʻo i le aiga subtilisin faʻatasi ai ma le faʻaogaina lelei o le enzymatic ma le lautele o le substrate specificity, lea e sili ona faʻaumatia noataga ester ma peptides e lata ane i le C-terminus o acids amino hydrophobic, amino acids o loʻo i ai le sulfur, ma fusi amino acids, lea e e masani ona faʻaaogaina e faʻaleagaina ai polotini e maua ai peptides pupuu. O le Proteinase K o loʻo i ai le uiga o le catalytic triad masani Asp39-His69-Ser224 tulaga ese i serine protease ma e lua Ca2 + faʻapipiʻi nofoaga i luga o le nofoaga autu e faʻateleina ai lona mautu ma faatumauina le maualuga o le enzymatic gaioiga i lalo o le lautele lautele o tulaga.
1. Pusa su'esu'ega fa'a kenera.
2. RNA ma DNA fa'apipi'i pusa.
3. Ave'ese vaega e le o ni polotini i totonu o aano ma fa'aleagaina mea leaga ole polotini (e pei ole tapenaga ole tui DNA ma le heparin).
4. Sauniuniga ole Chromosomal DNA mo Pulse Electrophoresis.
5. Fa'apalagi i Sisifo.
6. Atina'e ma le fa'atupuina o enzymatic glycated albumin reagents i le matata o in vitro diagnostics.
1. 30mg / fagu 1g / fagu poʻo 100g / fagu
2. 1kgs / taga pepa
Proteinase K ose serine protease mautu ma lautele substrate specificity. E fa'aleagaina ai le tele o polotini i totonu o le atunu'u e o'o lava i le i ai o ni vaila'au. E le o fa'agaoioia e u'amea u'amea, chelating agents (mo se fa'ata'ita'iga, EDTA), sulfhydryl reagents, po'o tripsin po'o chymotrypsin inhibitors. E mautu i luga o le lautele o le pH (4-12.5), ma le gaioiga sili ona lelei ile pH 6.5-9.5. Gaoioiga e mafai ona faaosofia e ala i le faʻaopopoina o mea faʻamalo (SDS ma Urea). Fa'atopetope denaturation o le enzyme e tupu i le vevela i luga atu o le 70°C. O le autolysis ole enzyme e tupu tele ile pH alkaline. Ae ui i lea, o le Proteinase K e le o atoatoa ona faʻaaogaina e le autolysis. O nisi vaega ole enzyme o lo'o fa'aauau pea ona fa'atumauina a latou gaioiga proteolytic atoatoa, tusa lava pe a uma le autolysis tele. O le Proteinase K e masani ona faʻaaogaina i le molecular biology applications e faʻafefe ai polotini e le manaʻomia, e pei o nucleases i DNA poʻo RNA sauniuniga mai meaola ninii, sela faʻaleaganuʻu, ma laau. O le enzyme e masani ona faʻaaogaina i le 50-200 ug / ml i sauniuniga nucleic acid ile pH 7.5-8.0 ma le 37 ~ 55oC. Taimi fa'a'a'ai e eseese mai le 30 minute i le 18 itula.
O se protease serine mautu ma maualuga le gaoioi; fa'aaogaina mo le protein ma le nucleic acid isolation.Proteinase K o lo'o fa'aaogaina i le fa'amamāina o le RNA ma le DNA mai masini po'o laina sela.
Matou te masani ona faʻatinoina le avea ma se tagata faigaluega faʻapitoa ma mautinoa o le a matou avatu ia te oe le sili sili ona aoga faʻatasi ai ma le tau faʻatau sili atu mo le Professional Design High Quality Proteinase K CAS Nu. 39450-01-6 ma le Tau Sili, Mo faʻamatalaga faaopoopo ma mea moni, faʻamolemole tautala. ia i matou i le vave e mafai ai!
Fuafuaga FaapolofesaSaina Tritirachium Album Limber ma Molecular Biology, Pulea lelei maoti e faʻatinoina i soʻotaga taʻitasi o le gaosiga atoa o le gaosiga. Matou te faʻamoemoe faamaoni e faʻavae le felagolagomai faauo ma fefaʻasoaaʻi faʻatasi ma oe. Faʻavae i luga o fofo maualuga ma atoatoa lelei muamua-faʻatau / ina ua uma-faʻatau auaunaga o lo matou manatu, o nisi tagata faʻatau na galulue faʻatasi ma i matou mo le silia ma le 5 tausaga.
